663 Specific polycyclic aromatic hydrocarbons elicit differential expression of CYP1A1 in trophoblast cells

Prenatal exposures to polycyclic aromatic hydrocarbons (PAHs) are associated with adverse outcomes including small for gestational age infants. We have previously reported differential levels of PAHs in the placenta preterm delivery. found that a specific PAH, dibenzoanthracene (DBA), was significantly higher than either benzo[a]pyrene (BaP) or Benzo[b]fluoranthene (BbF). Furthermore, we placental DBA and negatively correlated at In this study aimed determine if there is response trophoblast cells distinct PAH treatments. Immortalized choriocarcinoma (BeWo) were treated 10 μM BaP, BbF 24 hours. DMSO treatment served as control. Media collected measured hCG production using an ELISA assay. RNA extracted from after hours treatment, used make cDNA. TaqMan primers probes measure expression CYP1A1, gene involved processing xenobiotic compounds, RT-qPCR. GAPDH While each increased CYP1A1 compared DMSO, elicited largest response, 54-fold increase (p<0.001). 16.5-fold (p=0.001), 7.4-fold BaP (p=0.006). Levels not altered any consistent previous publications cells. tissue, BbF, placentae birth. find elicits upregulation BbF. Further experimentation necessary RNA-Seq analyses help yield insight into pathways differentially responsive DBA, yielding its association